Tag Archives: lxr agonists

br Materials and methods br Discussion This was a

Materials and methods

This was a hospital-based study investigating the prevalence and predictors of AD in men with LUTS in a medical center in Taiwan. We found that the prevalence of AD was estimated to be 55.7% in men with LUTS in our Urology Outpatient Clinic. There was a considerable portion of patients both with LUTS and AD that had hyperlipidemia, pre-DM, coronary artery disease, and lxr agonists (23.5%, 48.5%, 4.4%, and 46.3%, respectively). Although there was no statistical difference in the prevalence of these associated factors compared with those of the non-AD group, we still need to pay attention to these baseline conditions to identify potential AD patients. As for the other parameters, elevated WBC and WC were significantly associated with AD and increased body weight was borderline associated with AD in the univariate analysis. In the multivariate analysis, however, only men with LUTS with elevated WC revealed a significantly higher risk for AD, and elderly men with LUTS showed a borderline risk for AD. Men with LUTS and WC > 90 cm had a 2.86-fold higher risk for AD than those who had WC < 90 cm. Given that some previous studies found a positive association between metabolic syndrome and LUTS, while other studies reported conflicting data, we only analyzed the parameters in metabolic syndrome (including hyperglycemia, hypertension, triglycerides, high density lipoprotein-C, WC) separately and determined that WC can be seen as an indicator of AD in men with LUTS in Taiwan. Although previous studies have investigated the relationship between AD and LUTS, no consistent correlations were found. In the Third lxr agonists National Health and Nutrition Examination Survey, Rohrmann et al evaluated the association of circulating sex steroid hormone with LUTS. No consistent associations were observed between circulating and free testosterone and risk of LUTS. In Kim et al\’s investigation of the relationship between nocturia and decreased serum testosterone in men with LUTS in Korea, decreased testosterone was a significant independent risk for overall nocturia, especially nocturnal polyuria, and patients with low serum testosterone showed increased nocturnal urine output. Yassin and colleagues proposed the potential role of testosterone in the urinary tract in that testosterone may interact with LUTS via several possible mechanisms, such as by acting on nonpost synaptic receptors in the bladder detrusor muscle and by stimulating nitric oxide production in the urinary tract and bladder. Despite the inconsistencies so far observed in the relationship between AD and LUTS, replacement with testosterone for men with late-onset hypogonadism (LOH) improves LUTS. Testosterone therapy improves LUTS/bladder function by increasing bladder capacity and compliance, and decreasing detrusor pressure in men with LOH. One 5-year prospective, observational study also demonstrated that testosterone replacement is associated with improvements in LUTS and International Prostate Symptom Score in men with LOH. These studies indicate the importance of identifying AD in men with LUTS, with early detection and replacement of testosterone for AD being able to improve the life quality in men with LUTS.
In our study, increased WC rather than body mass index is an independent indicator of AD and in men with LUTS. One epidemiological investigation by Svartberg et al enrolled 1548 men aged 25–84 years surveyed the correlation between sex steroid hormones and central obesity. They found that all hormone associations were stronger for WC than for body mass index and suggested that WC should be the preferred anthropometric measurement in predicting endogenous testosterone levels. Measuring WC is a simple way to predict intra-abdominal fat volume or area and where it is placed around the body and increased intra-abdominal fat is associated with an increase in insulin resistance and systemic inflammation, which may contribute to low testosterone levels. In addition, total and free testosterone levels have been observed to decrease with increasing age in longitudinal studies, and thus it is not surprising in our study that older age was associated with a borderline rise in the risk of AD.

Despite the physical distance between the domestic pigs

Despite the physical distance between the domestic pigs and the populations of other animals in this study, neutralizing antibody against WBRV1 was detected in a domestic pig serum. In Japan, wild boars represent a constant threat for introducing the viruses, such as Aujeszky’s disease virus, into the commercial swine industry (Mahmoud et al., 2011). Therefore, wild boars may play a role in transmission WBRV1 into swine population in Japan and WBRV1 may have already infiltrated Japanese pig farms. However, the prevalence of WBRV1 in swine population in Japan should not be estimated by only serological data. Further studies are needed to clarify the prevalence of this virus in pigs. In this study, we did not investigate how WBRV1 infects animals. Thus, understanding of the prevalence of this virus in not only pigs but also other animals and insects and exploration of its life cycle needs additional investigations.

Conflict of interest


The Alloherpesviridae is a new virus family in the Herpesvirales order. It is comprised of both piscine and amphibian herpesviruses (McGeoch et al., 2006) and is evolutionary distinct from the other families of the order Herpesvirales (Davison et al., 2009; Hanson et al., 2011; Waltzek et al., 2009). Members of the Alloherpesviridae family are increasingly recognised as pathogens in aquaculture. One important pathogen is the Cyprinid herpesvirus 3 (CyHV-3), a herpesvirus from the Cyprinivirus genus which infects common carp, Cyprinus carpio and its coloured variety, the koi (Waltzek et al., 2009). CyHV-3 infections may cause severe outbreaks of the so called koi herpesvirus disease (KHVD) leading to up to 100% mortalities in infected populations, consequently causing a severe negative impact on carp aquaculture and the ornamental koi trade.
Characteristic features of the virus include a large 295kbp long linear genome with 156 potential ORFs (Aoki et al., 2007) encoding for at least 40 proteins building the mature viron (Michel et al., 2010). The CyHV-3 virion has an icosahedral capsid, an amorphic protein tegument and a lipid envelope containing virus glycoproteins (Dishon et al., 2005; Hutoran et al., 2005) which it acquires during the lxr agonists step from infected cells. This process is similar to the mechanism observed for mammalian herpesviruses (Mettenleiter, 2002). In electron microscopical studies on infected cells, CyHV-3 nucleocapsids appear to bud from the inner nuclear membrane into the perinuclear space. When the nucleocapsids cross the outer nuclear membrane into the cytoplasm, its primary envelope is lost and a second envelope is acquired through budding into cytoplasmic vesicles (Hanson et al., 2011; Miwa et al., 2007).
In enveloped viruses, the viral membrane is required for the critical steps of entry into the target cell and fusion with the host’s cellular membrane in order to deliver the viral capsid into the cell cytosol. The classical endocytotic pathway, which many viruses use as the primary step of internalisation, is clathrin-dependent endocytosis (Marsh and Helenius, 2006). Instead, many viruses use the lipid raft/caveola-dependent entry route, which is characterised by the importance of high levels of cholesterol and sphingolipids ((Doherty and McMahon, 2009) and references therein). In the plasma membrane of cells (chole) sterol–sphingolipids are particularly enriched in dynamic nanoscale liquid ordered microdomains, ordered assemblies of proteins and lipids, which are also termed as lipid rafts (Simons and Toomre, 2000). These microdomains are associated with a range of important cellular and physiological functions including cell signalling, membrane and protein trafficking and sorting and nutrient transport. Lipid rafts have been extensively studied in mammals. In fish, lipid rafts have been isolated from rainbow trout (Zehmer and Hazel, 2003), Atlantic cod (Gylfason and Asgeirsson, 2008), and common carp (Brogden et al., 2014), furthermore the presence of functional lipid rafts in goldfish macrophages was shown (Garcia-Garcia et al., 2012). The lipid raft model is used to investigate a large range of processes including, protein trafficking (Ikonen, 2001), metabolic diseases (Maalouf et al., 2010) and cell signalling (Magee et al., 2002; Varma and Mayor, 1998). In addition, lipid rafts and cell membrane cholesterol were found to be involved in various stages of the viral life cycle, such as entry (Nguyen and Hildreth, 2000; Ono and Freed, 2001), assembly and budding (Chazal and Gerlier, 2003). Many studies demonstrated that several enveloped viruses enter host cells in a cholesterol-dependent manner, including coronavirus (Choi et al., 2005), poxvirus (Chung et al., 2005), paramyxovirus (Martin et al., 2012) and herpesvirus (Bender et al., 2003).